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TransDetect? Cell Counting Kit (CCK)

細(xì)胞增殖及細(xì)胞毒性檢測(cè)試劑盒

目錄號(hào)規(guī)格單價(jià)
FC101-011 ml220
FC101-025 ml720
FC101-032×5 ml1290
FC101-046×5 ml3080
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產(chǎn)品詳情介紹

TransDetect? Cell Counting Kit,是一種基于水溶性四唑鹽的細(xì)胞增殖和細(xì)胞毒性檢測(cè)試劑盒。它在電子耦合試劑1-Methoxy PMS存在的情況下,可以被線粒體內(nèi)的脫氫酶還原為可溶性的橙黃色甲臜(formazan)。甲臜的數(shù)量與活細(xì)胞的數(shù)量成正比。細(xì)胞增殖越快、細(xì)胞毒性越小、細(xì)胞數(shù)量越多,則顏色越深,顏色的深淺與細(xì)胞數(shù)量呈現(xiàn)良好的線性關(guān)系。該產(chǎn)品細(xì)胞毒性小,對(duì)后續(xù)實(shí)驗(yàn)沒(méi)有影響,與MTT,XTT,MTS和WST-1相比,此法檢測(cè)靈敏度更高,線性范圍更寬,適用于藥物篩選,細(xì)胞增殖測(cè)定,細(xì)胞毒性測(cè)定和腫瘤藥敏實(shí)驗(yàn)。 

產(chǎn)品組成

image.png

實(shí)驗(yàn)數(shù)據(jù)

CCK靈敏度檢測(cè)

image.png


CCK對(duì)細(xì)胞毒性低

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References

1 Zhao J, Chu H, Zhao Y, et al. A NIR light gated DNA nanodevice for spatiotemporally controlled imaging of microRNA in cells and animals[J]. Journal of the American Chemical Society, 2019.(IF 14.69)

2 Ma M, Cai B, Zhou Z, et al. LncRNA-TBP mediates TATA-binding protein recruitment to regulate myogenesis and induce slow-twitch myofibers[J]. Cell Communication and Signaling, 2023.(IF 7.52)

3 Cai B, Ma M, Zhang J, et al. Long noncoding RNA ZFP36L2-AS functions as a metabolic modulator to regulate muscle development[J]. Cell Death & Disease, 2022.(IF 8.46)

4 Zhong F, Liu J, Gao C, et al. Downstream regulatory network of MYBL2 mediating its oncogenic role in melanoma[J]. Frontiers in Oncology, 2022.(IF 6.24)

5 Zhao M M, Zhu Y, Zhang L, et al. Novel cleavage sites identified in SARS-CoV-2 spike protein reveal mechanism for cathepsin L-facilitated viral infection and treatment strategies[J]. Cell Discovery, 2022.(IF 38.07)

6 Liao N, Su L, Zheng Y, et al. In vivo tracking of cell viability for adoptive natural killer cell‐based immunotherapy by ratiometric NIR‐II fluorescence imaging[J]. Angewandte Chemie, 2021.(IF 15.33)

7 Liao N, Su L, Cao Y, et al. Tracking cell viability for adipose-derived mesenchymal stem cell-based therapy by quantitative fluorescence imaging in the second near-infrared window[J]. ACS nano, 2022.(IF 15.88)


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