產(chǎn)品詳情介紹
CD73又名胞外-5′-核苷酸酶（NT5E），是一種跨膜糖蛋白，通過糖基磷脂酰肌醇（GPI）錨定于細(xì)胞膜，將細(xì)胞外的核苷酸水解為膜可滲透核苷[1]。CD73在淋巴細(xì)胞、間充質(zhì)干細(xì)胞、濾泡樹突狀細(xì)胞、內(nèi)皮細(xì)胞和上皮細(xì)胞亞群等中均有表達(dá)，協(xié)同刺激T細(xì)胞的活化并調(diào)節(jié)淋巴細(xì)胞與內(nèi)皮細(xì)胞的粘附[2-5]。CD73被用作淋巴細(xì)胞分化的標(biāo)志物，因此，各種免疫缺陷疾病中都會(huì)出現(xiàn)CD73缺陷[4,6]。本產(chǎn)品為小鼠抗人CD73單克隆抗體，用于WB、IP、IF和FC方法特異性檢測(cè)人CD73蛋白。
WB: ProteinFind? Anti-CD73 Mouse Monoclonal Antibody 檢測(cè) MDA-MB-231 細(xì)胞中 CD73 蛋白的表達(dá)。預(yù)測(cè)分子量: 63 KDa，實(shí)際分子量: 70 KDa,一抗稀釋倍數(shù): 1:1000
WB: ProteinFind? Anti-CD73 Mouse Monoclonal Antibody 檢測(cè) Hep G2(陰性細(xì)胞)、U251(陽性細(xì)胞)、HeLa(陰性細(xì)胞)、K-562(陰性細(xì)胞)、Huh7(陰性細(xì)胞)、MDA-MB-231(陽性細(xì)胞)、MSC(陽性細(xì)胞)及HT-29(陽性細(xì)胞)中 CD73 蛋白的表達(dá)。預(yù)測(cè)分子量：63 KDa，實(shí)際分子量：70 KD,一抗稀釋倍數(shù): 1:1000
IP: ProteinFind? Anti-CD73 Mouse Monoclonal Antibody 對(duì) MDA-MB-231 細(xì)胞裂解液 IP 檢測(cè)結(jié)果。第一泳道為10 % Input，第二泳道所用抗體為ProteinFind? Anti-CD73 Mouse Monoclonal Antibody，第三泳道為鼠 IgG 陰性對(duì)照?？贵w稀釋倍數(shù)：1:100
IF: ProteinFind? Anti-CD73 Mouse Monoclonal Antibody (綠色)檢測(cè) MDA-MB-231 細(xì)胞 CD73 定位，Hoechst 用于標(biāo)記細(xì)胞核(藍(lán)色)。一抗稀釋倍數(shù): 1: 100
FC: ProteinFind? Anti-CD73 Mouse Monoclonal Antibody (綠色)對(duì) MDA-MB-231 細(xì)胞(陽性細(xì)胞)(A圖)、MSC細(xì)胞(陽性細(xì)胞)(B圖)和HeLa細(xì)胞(陰性細(xì)胞)(C圖)的 FC 檢測(cè)結(jié)果。陰性對(duì)照為 Mouse IgG1 Isotype Control (紅色)。一抗稀釋倍數(shù): 1: 100
CD系列產(chǎn)品介紹視頻
[1] Le Hir M, Kaissling B. Distribution and regulation of renal ecto-5'-nucleotidase: implications for physiological functions of adenosine [J]. Am J Physiol. 1993, 264(3 Pt 2): F377-87.
[2] Minor M, Alcedo KP, Battaglia RA, et al. Cell type- and tissue-specific functions of ecto-5'-nucleotidase (CD73) [J]. Am J Physiol Cell Physiol. 2019, 317(6): C1079-C1092.
[3] Tan K, Zhu H, Zhang J, et al. CD73 Expression on Mesenchymal Stem Cells Dictates the Reparative Properties via Its Anti-Inflammatory Activity [J]. Stem Cells Int. 2019: 8717694.
[4] Airas L, Jalkanen S. CD73 mediates adhesion of B cells to follicular dendritic cells [J]. Blood. 1996, 88(5): 1755-64.
[5] Airas L, Hellman J, Salmi M, et al. CD73 is involved in lymphocyte binding to the endothelium: characterization of lymphocyte-vascular adhesion protein 2 identifies it as CD73 [J]. J Exp Med. 1995, 182(5): 1603-8.
[6] Kim E-S, Ackermann C, Tóth I, et al. Down-regulation of CD73 on B cells of patients with viremic HIV correlates with B cell activation and disease progression [J]. Journal of Leukocyte Biology. 2017, 101(5): 1263-71.
