產(chǎn)品詳情介紹
本產(chǎn)品通過在重疊區(qū)域引入突變位點(diǎn)的方法，利用TransStart? FastPfu Fly PCR SuperMix 擴(kuò)增，制備包含突變的PCR片段；利用特殊的重組酶和同源重組原理，將不多于6個片段無縫拼接，從而實(shí)現(xiàn)多點(diǎn)突變。
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5 Zhang L, Wang L, Wu L, et al. Biochemical characterization and mutational studies of a thermostable endonuclease III from Sulfolobus islandicus REY15A[J]. International Journal of Biological Macromolecules, 2021.(IF 6.95)
